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Figure 2. Expression of MMPs from Emdogain-stimulated MG-63 cells. MG-63 cells (1 x 10⁶ cells) were incubated in serum-free media containing 50, 100, and 200 µg/mL of Emdogain for 24 hrs and in the conditioned media as described in MATERIALS & METHODS. (A) The concentrated media were separated on 8% SDS-PAGE, blotted with anti-MMP-1 antibody, and visualized with a Super Signal west pico chemiluminescent substrate. Molecular markers (kDa) are shown in the left column. Quantification of MMP-1 (upper panel) was performed densitometrically with NIH image software. The intensities of each band are depicted as percent of maximum value (lower panel). (B) The same conditioned media were subjected to gelatinzymography as described in MATERIALS & METHODS. As a standard, conditioned medium prepared from HT1080 cells stimulated with ConA was used to localize inactive (upper) and active (lower) forms of MMP-2 and are shown as lines. These data are representative of more than 3 independent experiments.

J DENT RES, Vol. 87, No. 8, 782-787 (2008)
DOI: 10.1177/154405910808700805

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