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Figure 3. Immunohistochemical (IHC) analysis of mandibular implant bioengineered tooth tissues. Bioengineered dental tissues were positive for amelogenin (AM) (A,B), dentin sialophosphoprotein (DSPP) (D,E), periostin (PER) (G,H), and vimentum (VM) (J,K). Corresponding immunostaining of natural tooth tissues is shown (C,F,I,L). All isotype control immunostaining was negative. Three experimental PGA and three experimental PLGA cell-seeded scaffold implants were analyzed by IHC. Two negative control ‘scaffold alone’ controls and 2 positive control tooth bud implants were also analyzed by IHC. Abbreviations: AM, amelogenin; DSPP, dentin sialophosphoprotein; PER, periostin; VM, vimentum. Scale bars = 200 µm.
J DENT RES, Vol. 87, No. 8,
745-750 (2008)
DOI: 10.1177/154405910808700811
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