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Figure 2. Radiographic and histological analysis of harvested and sectioned 12-week experimental and control implants. (A) Negative control implant site. (B) Localized radiopaque areas (arrows) indicate mineralized tissue formation at the cell-seeded implant site. (C,E) H&E-stained positive control implanted 4-dpn tooth buds exhibited well-formed tooth structures, with organized crown and root structures. (D,F) Goldner’s stained 4-dpn tooth bud implants revealed characteristic blue-stained dentin, and brown-stained immature enamel. (G,I) H&E-stained bioengineered dental tissues present in jaw implants. (H,J) Goldner’s stained sections revealed blue-stained dentin, brown-stained immature enamel, and gray-stained mature enamel tissues. Unseeded, negative control scaffolds did not form dental tissues (data not shown). All 16 of the experimental cell-seeded implants and all of the 14 implanted tooth bud controls produced radiopaque dental tissues. None of the negative control unseeded scaffolds produced radiopaque mineralized tissue. Abbreviations: am, ameloblasts; b, bone; d, dentin; e, enamel; ime, immature enamel; me, mature enamel; od, odontoblasts; p, pulp; pd, predentin. Scale bars are 100 µm.
J DENT RES, Vol. 87, No. 8,
745-750 (2008)
DOI: 10.1177/154405910808700811
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