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Figure 1. Dental cell proliferation in culture. (A) Analysis of cell number at day 0, and after 10 days in culture, revealed that enzyme concentrations of 0.4 mg/mL collagenase and 0.2 mg/mL dispase resulted in the highest cell yields. (B) Growth curves for digested tooth bud cell preparations confirmed that tooth buds digested with 0.4 mg/mL type I collagenase and 0.2 mg/mL dispase I exhibited the highest cell number after 10 days in culture. (C) Digital images of cultured tooth bud cells, prepared with the 0.4 mg/mL collagenase and 0.2 mg/mL dispase enzyme concentrations, after 2, 4, and 10 days in culture. Five independently isolated cell preparations were examined for each type of analysis (Table). Wilcoxon’s test revealed highly significant values of P = 0.0003. Scale bar is 2 mm.
J DENT RES, Vol. 87, No. 8,
745-750 (2008)
DOI: 10.1177/154405910808700811
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