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Figure 2. The expression of matrix metalloproteinase (MMP)-3 protein increased by compressive forces. (A) MG63 cells were exposed to a compressive force for 1 day, and then whole-cell lysate from cells was subjected to Western blot analysis with an antibody against MMP-3. Compared with the control group, the intensity of MMP-3 was greater in the compression group. (B) Culture medium was collected after 24 hrs, with or without compressive force stimulation, and incubated with heparin Sepharose beads to enrich MMP binding. The bound proteins were loaded and detected with an antibody to MMP-3. The 57- and 47-kDa bands correspond to the latent and active forms, respectively.
J DENT RES, Vol. 87, No. 7,
692-696 (2008)
DOI: 10.1177/154405910808700714
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