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Figure 3. NAC protects the human pulp from repeated insults by restorative materials. (A) Human pulp cells extracted from control teeth and those restored with composite in the presence and absence of NAC, as described in Fig. 2A, were grown, and the viable cells from each group (1 x 105) were treated with and without HEMA (0.0082 M) for 18–24 hrs, and the levels of cell death were determined by Annexin V and Propidium Iodide (PI) staining. The numbers in each histogram are the percentages of cells positive for that quadrant. (B) We obtained levels of cell death in each histogram from Fig. 3A by adding the percentages of cells positive in quadrants 1, 2, and 4.
J DENT RES, Vol. 87, No. 6,
537-541 (2008)
DOI: 10.1177/154405910808700603
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