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Figure 1. Comparisons of the tissue histology and mineralized tissue structure of fracture callus and distraction gap tissues. Murine femur fracture calluses and tibia distraction gap tissue were prepared from specimens obtained 21 days post-fracture or at 21 days post-surgery. (Panel A) Representative longitudinal sections of fracture and distraction osteogenesis were stained with Safranin-O/fast green. Original magnification 25x. (Panel B) Representative longitudinal microCT images at a resolution of 12 microns. Arrows indicate the extent of new bone formation. Both sets of images are presented with the distal and proximal orientations, left to right. The various zones in distraction osteogenesis are indicated. The central fibrous zone, histologically called the fibrous interzone (FIZ), is rich in chondrocyte-like cells, fibroblasts, and oval cells that are morphologically intermediate between fibroblasts and chondrocytes. The fibrous interzone contains differentiating osteoblasts that deposit osteoid along collagen bundles. When these collagen bundles mineralize, they form a zone called the zone of microcolumn formation (MCF). In between the fibrous interzone and the zone of microcolumn formation is a zone of high cell density called the primary matrix or mineralization front (PMF). Separate scale bars for both the histological and microCT images are presented below each image (1 mm).
J DENT RES, Vol. 87, No. 2,
107-118 (2008)
DOI: 10.1177/154405910808700215
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