|
Sign In to gain access to subscriptions and/or personal tools.
|
Click on image to view larger version.
Figure 2. Influence of Poly(P) on calcification and decalcification with in vitro analysis. MC 3T3-E1 cells were cultured with 1 mM Poly(P)75 or 1 mM NaPO4 for 25 days and stained by the von Kossa method. Arrow represents nodule formation (A). After co-culture of mouse calvarial osteoblasts and bone marrow macrophages, a pit formation assay in the cultivation with the dentin slice was performed. Dentin decalcified by osteoclasts was stained by Mayer’s hematoxylin (B) and counted microscopically (C). Bar: 5 µm. Error bars represent the mean ± SD of three independent analyses.
J DENT RES, Vol. 86, No. 9,
893-897 (2007)
DOI: 10.1177/154405910708600917
|
|
