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Figure 1. Expression levels of osteoblast-related genes by real-time polymerase chain-reaction and alkaline phosphatase activity in MC 3T3-E1 cells. Cells were cultured in differentiation medium with 1 mM Poly(P) or without 1 mM Poly(P) (Control) for 35 days. Relative mRNA levels of OPN (A), OCN (B), Col I (C), OPG (D), and ALP activity (E)—without treatment (open circles) and following treatment with 1 mM Poly(P) (closed circles)—are shown. Error bars represent the mean ± SD of three independent analyses.
J DENT RES, Vol. 86, No. 9,
893-897 (2007)
DOI: 10.1177/154405910708600917
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