|
Sign In to gain access to subscriptions and/or personal tools.
|
 |
Click on image to view larger version.
Figure 3. Protein expression of 3D constructs. (A,B) Photomicrographs of representative histological sections after immunohistochemical analysis for MMP-1 (A) and MMP-3 (B). The inset images show partial magnification. Bar = 100 µm. Intense dark brown staining of MMP-1 and MMP-3 is evident in all constructs. More immunopositive cells were observed in the 20-kPa-loaded constructs than in the non-loaded constructs. (C) Western blotting for the active form of MMP-2 in the 3D constructs. The expressions of 72-kDa and 62-kDa bands were higher in the 20-kPa-loaded blots than in other blots. As a positive control, the culture medium of HT 1080 fibrosarcoma cells was used. (D) Western blotting for MMP-9 in the 3D constructs. The expressions of 92- and 68-kDa bands were not detected. As a positive control, the culture medium of HT 1080 fibrosarcoma cells was used. (E) Gelatin zymography for pro-MMP-9, pro-MMP-2, and MMP-2 in the culture supernatants of 3D constructs. Three bands of gelatinolytic activity corresponding to molecular weights of 92, 72, and 62 kDa were detected in the culture medium of HT 1080 fibrosarcoma cells as the positive control, and 2 bands of gelatinolytic activity corresponding to molecular weightsec of 72 and 62 kDa were detected in the constructs.
J DENT RES, Vol. 86, No. 8,
786-791 (2007)
DOI: 10.1177/154405910708600819
|
|
