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Figure 3. Serum albumin as an alternative probe for wounding. (A) Undisturbed maxillary gingiva was immunostained with an antibody directed against mouse serum albumin. As was the case when fluorescein-labeled dextran was used as a cell wound probe, very little intracellular labeling of epithelial cells with this antibody is observed. (B) Brushed mandibular gingiva immunostained for serum albumin. In contrast to undisturbed gingiva, brushed gingiva displayed heavy labeling of surface epithelial cells. (C) Undisturbed tongue skeletal muscle was immunostained for serum albumin. Positively stained skeletal muscle fibers were rarely observed. (D) Brushed tongue was immunostained for serum albumin. Positively stained myofibers were abundant. (E) The same section of undisturbed gingiva was immunostained with an antibody against c-fos. As was the case in fluorescein-labeled dextran-treated tissues, very few positively stained nuclei were observed. (F) Corresponding immunostaining for c-fos. Numerous c-fos-positive nuclei were observed in brushed epithelium. (G) Corresponding immunostaining for c-fos. Myofiber nuclei, positively stained for c-fos, were not observed. (H) Corresponding immunostaining for c-fos. Positively stained myofiber nuclei were abundant. Magnification bar = 20 µm.
J DENT RES, Vol. 86, No. 8,
769-774 (2007)
DOI: 10.1177/154405910708600816
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