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Figure 1. Effect of IL-1 on the expression of COX-2 in odontogenic keratocyst fibroblasts. (A) A representative immunohistochemical staining for COX-2 in a section of an odontogenic keratocyst. Subepithelial layer fibroblasts were positively stained with anti-COX-2 antibody (arrowheads). Scale bar: 12 µm. (B,C) Fibroblasts (4 x 104 cells/cm2) were incubated in the absence (Co) or presence of various concentrations of rhIL-1 for 6 hrs (B), or were incubated with 0.1 nM rhIL-1 for various times (C). The expression of COX-2 mRNA was measured as described in "MATERIALS & METHODS". The amounts of COX-2 mRNA in the control were normalized as 1.0. Vertical bars indicate mean ± SD (n = 3). * Significant difference at p < 0.05. (D) Fibroblasts were incubated in the absence or presence of 0.1 nM rhIL-1 for 12 hrs, and the aliquots of cell lysates were subjected to Western immunoblotting for COX-2, as described in "MATERIALS & METHODS".
J DENT RES, Vol. 86, No. 2,
186-191 (2007)
DOI: 10.1177/154405910708600215
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