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Journal of Dental Research
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Figure 3


Figure 3. Immunohistology of marginal gingiva and periodontium of rat molar tooth. (A) Histology of marginal gingiva and root (HE stain). Root dentin (De) and the emerging fibrous tissue with gingival fibroblasts (GF) aligned, running toward the periosteum and up toward the papillae. Part of the epithelium (Ep) is seen next to the root. (B) Cross-section of a molar tooth and its periodontium, including papillae of gingiva and PDL. Positive immunostaining with P2X4 antibodies (black) is seen specifically in the fibrous connective tissue of the papillae and PDL only. Alveolar bone (AB), epithelium (Ep), and dentin (De) were negative. (C,D) The same slide from the marginal periodontium after coronal approach surgery (treatment). (C) Positive immunostaining (black) for P2X4 receptors in the marginal gingival connective tissue, mainly fibroblasts. (D) The same, but illuminated by UV, showing the fibroblasts. (E,F) Similar. (F) Poor immunostaining for P2X4 in control sections where apical surgery was performed, from the marginal gingiva. The scale bars represent 50 µm.

J DENT RES, Vol. 86, No. 2, 181-185 (2007)
DOI: 10.1177/154405910708600214





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