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Figure 3. Expression and immunostaining of LAMP-1 in human enamel organ epithelial cell cultures. (A) Reverse-transcription (RT)-PCR product showed positive LAMP-1 mRNA expression in human enamel organ epithelial (EOE) cells, indicated by a 513-bp band in 1% gel. Human enamel organ cDNA library was used as a positive (+) control, while 2 µL of sterile dH2O was used as a negative (–) control. (B) Immunofluorescence of enamel epithelial cells in vitro showed a weak positive staining for LAMP-1. (C) Pre-immune IgG staining of enamel epithelial cells showed immunonegativity for LAMP-1.
J DENT RES, Vol. 86, No. 11,
1095-1099 (2007)
DOI: 10.1177/154405910708601114
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