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Figure 2. Detection of LAMP-1 in the stratum intermedium of human enamel organ. (A) Immunofluorescent staining of LAMP-1 showed a strong positive staining for LAMP-1 in the stratum intermedium (SI) and stellate reticulum (SR) of a frozen section of a human enamel organ, while immunostaining for LAMP-1 in ameloblasts (Am) was not detectable. (B) Immunostaining of the human enamel organ showed that Notch1 was weakly positive in the SR, while it was hardly detectable in the SI and differentiated Am. (C) The positive control showed strong immunostaining for cytokeratin-14 in the SI and SR, but weaker staining in the Am. (D) The negative control stained with the pre-immune IgG was immunofluorescent-negative. Cell nuclei were counterstained with Hoechst dye (blue).
J DENT RES, Vol. 86, No. 11,
1095-1099 (2007)
DOI: 10.1177/154405910708601114
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