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Figure 2. PGE₂ regulated RANKL (A), OPG (B), and IL-6 (C) protein expression levels via EP4 in OCCM-30 cells. EP4 agonist, EP4A; EP4 antagonist, EP4R. After 36 hrs of incubation with PGE₂ (100 ng/mL) or the EP4 agonist (1 µM), with or without the EP4 antagonist (1 µM), culture media and cells were collected, and RANKL (cell lysate), OPG, and IL-6 (culture media) protein levels were measured by ELISA. Each result was normalized to total protein levels. RANKL protein level was markedly up-regulated by PGE₂ and the EP4 agonist (A). PGE₂ and the EP4 agonist significantly down-regulated OPG protein levels (B). IL-6 protein levels were significantly up-regulated by PGE₂ (C). The EP4 antagonist eliminated these effects of PGE₂. Results are expressed as the mean ± SD of 4 cultures. *p < 0.05 and **p < 0.01 vs. control.

J DENT RES, Vol. 86, No. 10, 974-979 (2007)
DOI: 10.1177/154405910708601011

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