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Figure 3. The number of TRAP-positive cells and the pit formation on dentin slices on the addition of conditioned medium or by co-culture of osteoclast precursor cells and compressed PDL cells. (A,B) Human osteoclast precursor cells cultured in commercial medium (-MEM supplemented with 10 unit/mL of penicillin, 10 µg/mL of gentamicin, and 10% fetal calf serum) with both macrophage colony-stimulating factor (M-CSF) and RANKL (both at 10 ng/mL) for 8 days. After the osteoclasts matured, the medium was changed to that obtained from human PDL cells after incubation with or without compressive-force-conditioned medium (2.0 g/cm²) for 12 hrs. The numbers of TRAP-positive cells (A) and resorption pits (B) were significantly increased by the conditioned medium (with compressive force) as compared with those in the respective controls (without compressive force). Further, the numbers of TRAP-positive cells and resorption pits were more abundant in the severe root resorption group (with compressive force) than in the non-resorption group (with compressive force). (C) Co-culture with compressed PDL cells (2.0 g/cm², 12 hrs) activated the differentiation of osteoclast precursor cells into mature osteoclasts for 8 days. The number of TRAP-positive cells in the compressed PDL cells was significantly differenr from that in the non-compressed PDL cells in both the severe root resorption and non-resorption groups. Furthermore, the increase was greater in the severe root resorption group than in the non-resorption group. We measured the numbers of TRAP-positive cells and resorption pits, and subjected them to statistical analysis. Data are expressed as the mean ± SD of 5 independent cultures. Significantly different from the corresponding control (*p < 0.001). Significantly different from non-resorption (with compressive force) (#p < 0.001).

J DENT RES, Vol. 85, No. 8, 751-756 (2006)
DOI: 10.1177/154405910608500812

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