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Figure 3. Arachidonic-acid-activated TREK-1 K⁺ channel opening. (A) Current traces from an inside-out patch with high-K⁺ bath solution containing arachidonic acid at various concentrations. The pipette solution was also the high-K⁺ solution. V_m = 20 mV. (B) Dose-dependent effects of arachidonic acid on the activity of the TREK-1 K⁺ channel (means ± SD, n = 8). The channel activity is expressed as the percentage of NP_o at each concentration relative to NP_o at 100 µM of arachidonic acid (%NP_o). NP_o values were obtained from current recordings in patches where the concentration of arachidonic acid in bath solution was altered between 0 µM and 100 µM by perfusion. Abscissa, common logarithmic scale. (C) %NP_o when K⁺ channel blockers were applied to the bath solution in inside-out patches in which TREK-1 K⁺ channels had been activated by 10 µM arachidonic acid. Ba²⁺ at a rather high concentration (3 mM) decreased channel activity. Quinine and quinidine were more effective than Ba²⁺. Each value was obtained from five inside-out patches (mean ± SD, n = 5). *Significantly different from blocker-free state.

J DENT RES, Vol. 85, No. 7, 664-669 (2006)
DOI: 10.1177/154405910608500716

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