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Figure 2. Apoptosis detection by the TUNEL method with compressive force application for 24 hrs in MG-63 cells. The apoptotic cells among MG-63 cells under compressive force were detected by the TUNEL method. Stained nuclei were observed in some cells under compressive force, but no stained nuclei were seen in control cells. (A) Control, magnification, x200; (B) cells under 2.04 x 10–4 N/cm2 of compressive force, x200; (C) cells under 2.04 x 10–4 N/cm2 of compressive force, x400. Bar = 100 µm. (D) Percentage of apoptotic cells induced by compressive force in MG-63 cells. Values represent the mean ± SD of the relative absorbance of 3 experimental samples (exp.) per control sample (cont.). Percentages were significantly different from controls at all forces except 1.02 x 10–4 N/cm2. At 2.04 x 10–4 and 4.08 x 10–4 N/cm2 of compressive force, the percentage of apoptotic cells increased between 12 and 24 hrs. The percentage of apoptotic cells increased in a force-dependent manner. Significantly different from controls: * p < 0.05 (Bonferroni/Dunn method); a, vs. control; b, vs. 1.02 x 10–4 N/cm2; c, between 12 hrs and 24 hrs (n = 3).
J DENT RES, Vol. 85, No. 3,
240-244 (2006)
DOI: 10.1177/154405910608500307
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