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Figure 1. Relative abundance of amelogenin transcripts containing exon 7 vs. those containing exons 8 & 9 and alignments of duplicated genome region with separate alignment of exon 5 with exon 8. (A) Quantitative real-time PCR analysis of amelogenin expression in first molars extracted from mice of the indicated age. Note that amelogenin transcripts with exon 7 were significantly more abundant than transcripts with exons 8 and 9. The error bars represent the standard error of the mean from 6 separate samples. EF1'1 expression served as the internal reference gene control, so the data were plotted relative to its expression. (B) Alignment of mouse exon 4, intron 4, and exon 5 (X4&5) with mouse exon 4b, intron 4b, and exon 8 (X4b8). The first underlined sequences denote exons 4 and 4b; the second underlined sequences denote exons 5 and 8. Nucleotide differences are italicized and highlighted in bold. Note the very high sequence identity starting from before exon 4 and extending past the aligned exons 5 and 8 sequence. Of 209 nucleotides, 191 are identical (91%), and all align without gaps. (C) Alignment of mouse exon 8 amino acid sequence (mouse 8) with exon 5 sequence from the indicated species. Amino acid differences are italicized and highlighted in bold. Note that when mouse exon 8 is compared with exon 5, just 8 nucleotide differences result in the encoding of 7 different amino acids between exons 5 and 8.
J DENT RES, Vol. 85, No. 10,
894-899 (2006)
DOI: 10.1177/154405910608501004
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