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Figure 1. OPG gene expression in the dental follicle of the first mandibular molar, showing the comparison of normals (N) vs. tl/tl mutants at different post-natal days. Comparisons are within a given day, because pups at different days are not littermates. For a given day, 3–4 pairs of littermates were analyzed. (A) Agarose gel picture from electrophoresis of semi-quantitative RT-PCR. (B) OPG gene expression ratio from semi-quantitative RT-PCR as normalized to their actin controls. (C) Real-time PCR showing gene expression of the mutants relative to that of their littermate controls, which was set as 1. Note that at all days except day 5, OPG gene expression is higher in the tl/tl mutants, and that for days 3, 7, and 11, this expression is significantly different (*) from the controls at P  0.05 by Student’s t test. Relative gene expression was calculated by the Delta CT method, which was normalized to the endogenous control of β-actin. Error bars in both B and C represent standard deviations (SDs).
J DENT RES, Vol. 84, No. 9,
837-841 (2005)
DOI: 10.1177/154405910508400911
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