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Figure 3. Dspp- and amelogenin-expressing cell layers from 2nd pharyngeal arches treated with BMP4. (A) In situ hybridization of section in (B) with Dspp. Expression is localized to an internal layer of cells. (C) Higher magnification of a region in (B) close to cartilage (blue arrowhead). Dashed lines outline the cells expressing Dspp. (D) Higher magnification of region in (C) of Dspp-expressing cells (blue arrow). Note the elongated shapes of the cells. (E) In situ hybridization of section in (F) with amelogenin. Expression is restricted to a layer of externally located cells. (G) Higher magnification of region in (F) adjacent to cartilage (blue arrowhead). Dashed lines indicate the cells expressing amelogenin. (H) Higher magnification of area in (G) showing cells expressing amelogenin (blue arrow). Note that the cells are round, obviously different from the Dspp-expressing cells. (I) Section of tissue formed following renal transfer of second-pharyngeal-arch explants treated with BSA beads. (J) In situ hybridization of section in (I) for Dspp expression. (K) In situ hybridization of section in (I) for amelogenin expression. Scale bars: 80 µm (A,B,E,F,I-K), 20 µm (C,G), and 5 µm (D,H).

J DENT RES, Vol. 84, No. 7, 603-606 (2005)
DOI: 10.1177/154405910508400704

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