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Figure 4. AP-1 activity of human gingival fibroblasts treated with and without 10 µg/mL cyclosporin A and 10 ng/mL TNF- or PDGF. (A) Gel shift assay; 5 µg of nuclear proteins were incubated with labeled [32P]ATP-labeled oligonucleotide probe, separated, and visualized by autoradiography, as described in the "METHODS". For competition, unlabeled nucleotide was added at 100-fold molar excess. Arrows indicate shifted complexes. (B) Luciferase reporter activity normalized for β-actin Renilla luciferase control. Mean ± SD of triplicates is shown (p < 0.025).
J DENT RES, Vol. 84, No. 6,
532-536 (2005)
DOI: 10.1177/154405910508400609
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