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Figure 2. Western blot analysis of immunoprecipitates obtained with the use of anti-p38 antibody. Human gingival fibroblasts were treated with 10 ng/mL TNF- and 10 µg/mL cyclosporin A. Cell lysates were subjected to immunoprecipitation, separated by SDS-polyacrylamide gel electrophoresis, and transferred to nitrocellulose membranes. Blots were probed with antibodies to phosphorylated (top panels) and unphosphorylated (bottom panels) enzymes. Incubation with TNF- was for 20 min (lanes "a" and "b") or for 4 hrs (lanes "c" and "d").
J DENT RES, Vol. 84, No. 6,
532-536 (2005)
DOI: 10.1177/154405910508400609
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