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Figure 1. Gel shift assay to determine DNA-binding activity of NFAT and NF B transcription factors. Nuclear extracts were obtained from human gingival fibroblasts exposed to 10 ng/mL TNF- in the presence or absence of 10 µg/mL cyclosprin A. Five µg of nuclear proteins were incubated with [32P]ATP-labeled oligonucleotide probes, separated as described in the "METHODS", and visualized by autoradiography. For competition, unlabeled nucleotides were added at 100-fold molar excess. Arrows indicate shifted complexes. A, NFAT; B, NFB.
J DENT RES, Vol. 84, No. 6,
532-536 (2005)
DOI: 10.1177/154405910508400609
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