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Figure 1. Model structure and PCR products of porcine amelogenin. (A) Model showing the intron-exon structure of the porcine amelogenin genes. The lines correspond to introns and the bars to exons. The white bars indicate the regions with the same sequence; arrows indicate oligonucleotide annealing sites. (B) Polyacrylamide gel (4.5%) stained with ethidium bromide showing PCR products observed when amplifying cDNA template and using amelogenin exon 1- and exon 7-specific primer sets. (C) PCR products observed when amplifying cDNA template and using amelogenin exon 5- and exon 7-specific primer sets. Lane M is the molecular marker (HpaI digest of x174 phage). (D) Agarose gel (1%) stained with ethidium bromide showing PCR products observed when amplifying genomic DNA with amelogenin exon 1- and exon 2-specific primer sets. The amplification products were separated on 1%. Lane M is molecular marker (HindIII digest of phage).

J DENT RES, Vol. 84, No. 2, 144-148 (2005)
DOI: 10.1177/154405910508400207

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