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Figure 4. Runx2 knockout (C,F,I,L,O) and Runx2/Runx3 double-knockout mice (G,J,M,P) exhibit prominent lingual epithelial buds in the upper but not the lower molars. (A,D) E13 wild-type, (B,E,H,K,N) E14 wild-type, (C,F,I,L,O) E14 Runx2 knockout, and (G,J,M,P) Runx2/Runx3 double-knockout. (A–C) Freshly dissected upper molars observed in the dissection microscope outlined with yellow dashed lines. Arrows indicate the location of sections stained with hematoxylin and eosin in D–F. Shh (H,J), Gli1 (I), Ptc1 (K–M), and Ptc2 (N–P) transcripts are expressed intensely in the lingual epithelial buds in Runx2 knockout (I,L,O) and Runx2/Runx3 double-knockout (J,M,P) upper molars. (Q–R) E14 Runx2 heterozygous mice also exhibit a prominent lingual bud at the anterior region of the upper molars. (S) There is intense Shh expression in the lingual epithelial bud (arrow) of Runx2 heterozygotes. Shh is also expressed intensely at the tip of the tooth bud, indicating the initiation of the enamel knot. (T) The cap-stage first molar tooth germ intensely expresses Shh in the enamel knot. Scale bar: 200 µm (D–G, Q); 100 µm (H–P, R–T).
J DENT RES, Vol. 84, No. 2,
138-143 (2005)
DOI: 10.1177/154405910508400206
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