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Figure 3. Biochemical analyses of pulp and periodontal ligament specimens (A-C). (A) mRNA expression of NPP1, ANK, TNSALP, collagen I- 1, and osteonectin relative to β2-microglobulin in periodontal ligament (open bars) and pulp (dashed bars) from normal teeth (mean ± SD; n = 14 for all datasets). (B) Activities of NPP1 (substrates: p-nitrophenyl phenylphosphonate and p-nitrophenylthymidine 5-monophosphate, respectively) and TNSALP (substrate: p-nitrophenylphosphate) in periodontal ligament (open bars) and pulp (dashed bars) of normal teeth (n = 20). Activities are given as mU/µg DNA. (C) Concentration of PPi relative to DNA content in periodontal ligament (open bar) and pulp (dashed bar) of normal teeth (n = 20). *p < 0.05; **p < 0.01; ***p < 0.001 according to the Wilcoxon signed-rank test.
J DENT RES, Vol. 84, No. 11,
1021-1025 (2005)
DOI: 10.1177/154405910508401110
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