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Figure 1. Sampling of dentin. (A) ± 100-µm-thick longitudinal sections were used for LM, EM, and biochemical analyses. (B) Dentin was sampled at 4 zones: crown (C) and root (R1-3). Samples were taken by means of a dental bur (1 mm diameter) running at low speed to prevent overheating. For microradiography, 200-µm-thick transverse sections were prepared (dashed lines). (C) Mineral density was determined at sites 1–4. P = pulp; E = enamel.
J DENT RES, Vol. 84, No. 11,
1021-1025 (2005)
DOI: 10.1177/154405910508401110
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