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Figure 1. Structure of N-linked glycans of IgG and analyses of IgG galactose deficiency. (A) Example of complex type N-linked glycans. Abbreviations: N-acetylglucosamine (GlcNAc), galactose (Gal), sialic acid (SA), mannose (Man), fucose (Fuc), asparagine (Asn). Variably present saccharides are in parentheses. Fc region-associated glycans of IgG are usually not sialylated, and only some of them are fully galactosylated or with bisecting N-acetylglucosamine. Galactose-deficient IgG glycans are terminated by N-acetylglucosamine residues that react with PVL lectin, while fully galactosylated glycans do not react with PVL. (B) Correlation between PVL reactivity (OD 405 nm, 30 min) and gas-liquid monosaccharide composition (mannose/galactose ratio) of differently galactosylated purified IgGs. Pierson’s correlation coefficient, r = 0.73 (N = 18).
J DENT RES, Vol. 84, No. 10,
897-901 (2005)
DOI: 10.1177/154405910508401005
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