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Figure 1. The effect of TEGDMA on the induction of apoptosis and necrosis in human primary pulp cells. Cell cultures were incubated with annexin V-FITC (apoptotic cells) and PI (necrotic cells) and analyzed by flow cytometry. The representative dual-parameter fluorescence histograms were derived from cell cultures exposed to various TEGDMA concentrations for 24 hrs. The numbers indicate the percentages of the viable (annexin V-; PI-) cell population (lower left quadrant), the apoptotic (annexin V+) cell population (lower right quadrant), and the necrotic cell populations in the upper right (annexin V+; PI+) and the upper left quadrants (annexin V-; PI +) in one typical experiment.
J DENT RES, Vol. 83, No. 9,
703-707 (2004)
DOI: 10.1177/154405910408300909
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