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Figure 2. Dependence of Ca-ATPase activity on pH. SR vesicles (0.1 mg/mL) from masseter (•), medial pterygoid ( ), and fast muscles ( ) were incubated at 37°C in media containing 50 mmol/L MOPS-Tris (pH 6.5–8.5) or MES-Tris (pH 5.0–6.4), 3 mmol/L ATP, 0.1 mmol/L CaCl2, 0.1 mmol/L EGTA, 3 mmol/L MgCl2, 100 mmol/L KCl, and 10 µmol/L calcimycin. The reactions and the Ca-ATPase activity determinations were performed as indicated in MATERIALS & METHODS. Bars, SD; n = 5.
J DENT RES, Vol. 83, No. 7,
557-561 (2004)
DOI: 10.1177/154405910408300709
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