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Figure 1. ATPase activity of SR membranes from masticatory muscles. SR vesicles (0.1 mg/mL) were incubated at 37°C in media containing 50 mmol/L MOPS-Tris (pH 7.2), 3 mmol/L ATP, 3 mmol/L MgCl2, 100 mmol/L KCl, and 10 µmol/L calcimycin. No Ca2+ was added. The reactions and the ATPase activity determinations were performed as indicated in MATERIALS & METHODS. Error bars indicate SD; n = 4. (A) ATPase activity as a function of EGTA concentration. SR vesicles from masseter (•) and medial pterygoid ( ) muscles were incubated in the above medium with EGTA added as indicated in the abscissa. (B) ATPase activity in the presence of thapsigargin for masticatory muscles. SR vesicles from masseter and medial pterygoid muscles were incubated as described above in the presence of 0.1 mmol/L EGTA and with (hatched bars) or without (blank bars) 0.4 µmol/L thapsigargin.
J DENT RES, Vol. 83, No. 7,
557-561 (2004)
DOI: 10.1177/154405910408300709
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