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Figure 3. Immunolocalization of  vβ3 integrin and osteoadherin in odontoblasts differentiated in vitro. (a) Confluent cells in the middle of the culture showed strong membrane staining with the anti-vβ3 integrin antibody and moderate staining of the cytoplasm. (b) Cell body and process were clearly delineated in some cells with typical odontoblast morphology. (c) At the culture periphery, where newly polarized cells aligned to constitute a typical odontoblast layer, vβ3 staining was detected on the membrane of odontoblast cell bodies at the level of contact zones and intercellular junctions (arrows). (d) In extracts of odontoblast cell cultures, osteoadherin was biochemically identified as a 110-kDa molecule with the purified anti-peptide antiserum (reducing conditions). No immunoreactive band was observed when the anti-osteoadherin antibody was omitted. (e) Osteoadherin staining was detected intracellularly close to the nucleus in all odontoblasts in vitro, but not in the extracellular compartment. Bars: 100 µm (a), 50 µm (b,c), 75 µm (e).
J DENT RES, Vol. 83, No. 7,
552-556 (2004)
DOI: 10.1177/154405910408300708
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