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Figure 1. Expression of  v and β3 integrin subunit genes in human dental pulp. (a) Analysis of the whole pulp by RT-PCR revealed the presence of mRNAs for both integrin subunits. PCR products migrate to a position in good agreement with their predicted sizes (v, 305 bp; β3, 517 bp). Ethidium-bromide-stained 2% agarose gel. Digestion with restriction enzymes confirmed amplicon identity (not shown). M: molecular-weight markers (pUC Mix Marker 8, Fermentas Inc., Hanover, MD, USA). (b-e) Detection of v and β3 integrin transcripts by in situ hybridization. v and β3 integrin subunit mRNAs were present in mature odontoblasts (Od) (b,c) and in cells surrounding blood vessels (BV) (d,e), but not in pulp core cells (P). (f) Hybridization with the β3 integrin subunit sense probe. No signal was detected. Bars: 40 µm.
J DENT RES, Vol. 83, No. 7,
552-556 (2004)
DOI: 10.1177/154405910408300708
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