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Figure 2. Positive TRAP-immunoreactivity in stromal cells of ameloblastoma (A), dentigerous cyst (B), odontogenic cyst (C), and radicular cyst (D). Giant cell granuloma served as positive control (E), while the fibro-epithelial polyp served as negative control (F). TRAP-positive cells are recruited from blood vessels supplying the osteolytic lesions (G). Multinucleation of pre-osteoclasts to mature osteoclasts is demonstrated by immunofluorescence (H). Nuclei are stained red by propidium iodide, and TRAP-positive cells are stained green. In situ hybridization showing positive staining for human calcitonin receptor in two multi-nucleated cells (I) and a negatively stained control (J).
J DENT RES, Vol. 83, No. 4,
349-353 (2004)
DOI: 10.1177/154405910408300415
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