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Figure 1. Immunohistochemical classification of dental epithelial cells in the rat lower incisor. (A) Expression patterns of CK14, ALP, amelogenin, p75NGFR, and Notch2 in rat lower incisors. (a) Hemotoxylin/eosin staining. (b) Expression of CK14 was observed in the entire dental epithelium, but not in the mesenchymal cells. (c) ALP activity was expressed in the stratum intermedium (arrowheads) and odontoblasts (arrows). (d) Expression of amelogenin was detected in pre-ameloblasts and ameloblasts. (e) p75NGFR was strongly expressed in inner enamel epithelium (arrowheads). (f) Notch2 was expressed in the stellate reticulum and outer enamel epithelium. The region surrounded by white dots shows lingual epithelium. Asterisks show the location of the cervical loop. Bars = 200 µm. (B) Summary of the distribution of antigens in the rat incisor as shown by immunohistochemical analysis. The intensity of immunostaining is represented as follows: +weakly, ++moderately, +++strongly, and -negative.
J DENT RES, Vol. 83, No. 2,
129-133 (2004)
DOI: 10.1177/154405910408300209
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