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Figure 2. Experimental procedures. (A) Dermal fibroblast-seeded gelatin scaffold (Y component) is sandwiched between two microporous collagen sponges loaded with rhBMP2 (two X components). (B) Location of surgically created calvarial defect in the parietal bone in relation to the natural sagittal suture (s) and coronal suture (c). (C) Surgical creation of full-thickness calvarial defect with a dimension of 6 x 2 x 8 mm³ in the center of the rabbit parietal bone devoid of natural cranial sutures. The adjacent sagittal suture (s) is shown. The dura mater was kept intact. Following creation of surgical calvarial defects in the corresponding rabbits from which dermal fibroblasts had been obtained, composite tissue grafts were implanted into the center of the parietal bone devoid of natural cranial sutures (N = 3). In one control group, tissue grafts consisting of two rhBMP2-loaded collagen sponges without autologous fibroblasts were implanted in age- and sex-matched rabbits (N = 3). In another control group, tissue grafts consisting of two rhBMP2-loaded collagen sponges with an intervening fibroblast-free gelatin scaffold were implanted in age- and sex-matched rabbits (N = 3). (D) The surgically created calvarial defect was filled with a composite tissue construct consisting of autologous autologous fibroblast-seeded gelatin scaffold (Y component) that was sandwiched between two rhBMP2-loaded microporous collagen sponges (two X components). The adjacent sagittal suture (s) was not part of the surgically created calvarial defect.

J DENT RES, Vol. 83, No. 10, 751-756 (2004)
DOI: 10.1177/154405910408301003

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