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Figure 4. Injection of FIV(lacZ) into the right TMJ (5 x 106 infectious particles) resulted in successful transduction of primary sensory neurons with the reporter gene β-galactosidase in trigeminal ganglia ispilateral to the treated joint. (A) β-galactosidase expression was detected by X-gal histochemistry in sagittal sections of the right-side ganglion (4X), (B) primarily at its posterior and posterolateral regions (20X). (C) Injection of FIV( 'lac) did not result in β-galactosidase expression. (D) The X-gal staining was confirmed with immunocytochemistry, with the use of antibodies raised against bacterial β-galactosidase following FIV(lacZ) injection compared with (E) FIV('lac) treatment.
J DENT RES, Vol. 83, No. 1,
65-70 (2004)
DOI: 10.1177/154405910408300113
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