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Figure 2. Langerhans cells, dermal DCs, and B-cells contribute to the pool of CD83+ mature DCs in CP. Shown is double-immunofluorescence staining of representative CP gingival tissue. (A) Several immature CD1a+ (green, panel 1) DCs at the junction of epithelium and lamina propria co-express CD83 (yellow arrows, panel 3), suggestive of in situ maturation. Images were taken with the use of a 20X objective, at a final magnification of 200X. (B) Several DC-SIGN+ (green, panel 1) dermal DCs in the lamina propria co-express CD83 (yellow arrows, panel 3). Final magnification (optical and digital), 250X. (C) The vast majority of CD19+ B-cells (green, panels 1, 5, 9) and mature CD83+ DCs (red, panels 2, 6, 10) are single-positive; however, co-expression (yellow arrows, panels 3, 7, 11) is also evident. Final magnification (optical and digital): 200X (panels 1–4); 400x (panels 5–12).
J DENT RES, Vol. 82, No. 9,
736-741 (2003)
DOI: 10.1177/154405910308200915
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