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Figure 1. Distinct tissue compartmentalization of Langerhans cells and dermal DCs in chronic periodontitis (CP). Shown are double-immunofluorescence stainings of representative CP gingival tissue. (A) Clear compartmentalization of DC-SIGN+ (red) dermal DCs to lamina propria, Langerin+ (green) Langerhans cells to epithelium, with the complete absence of double-positive cells (yellow, merge). Images were acquired with use of the 20X objective, with a final magnification (optical and digital) of approximately 250X. Shown are the red (panel 1), green (panel 2), and merged (panel 3) channels of the image and the H&E-stained section (panel 4). (B) The vast majority of DC-SIGN+ cells (green) also express mannose receptor (MR) (yellow arrows, panel B3) and are distributed throughout the lamina propria. Images were taken with the use of a 20X objective, with a final magnification of 200X. Shown are green (panel 1), red (panel 2), and merged channels (panel 3) and a merge-DIC overlay (panel 4). Fluorescence images were acquired with a Nikon Eclipse 600 microscope equipped with a color high-resolution CCD camera and PC running Image Pro software. Images were sharpened with the use of 2D-deconvolution software.
J DENT RES, Vol. 82, No. 9,
736-741 (2003)
DOI: 10.1177/154405910308200915
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