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Figure 1. In situ detection of apoptosis. (A,B) Formaldehyde-fixed, paraffin-embedded healthy periodontal tissues of 4 molar teeth from 2 monkeys were analyzed by means of a TUNEL detection kit (Boehringer Mannheim). TUNEL-positive cells are arrowed. (C) The apoptotic cell percentage was obtained as the ratio of TUNEL-positive cells relative to the total number of counted fibroblasts. (D) The apoptotic cell density was expressed as TUNEL-positive cells per mm2. Mean ± SD (n = 12). **p < 0.01, t test, difference between PDLF and GF. Ce, cementum; PDL, periodontal ligament; AvB, alveolar bone; DG, deep gingiva.
J DENT RES, Vol. 82, No. 6,
454-459 (2003)
DOI: 10.1177/154405910308200610
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