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Figure 2. Photomicrographs of histologic phenotypes of a representative tissue-engineered mandibular condyle following 8 wks of in vivo implantation. (A) Von Kossa silver-stained section showing the interface between chondral and osseous layers. Multiple mineralization nodules were present in the osseous layer (lower half of the photomicrograph), but absent in the chondral layer (upper half of the photomicrograph). (B) Positive safranin O staining of the chondrogenic layer was represented by intense red, indicating the synthesis of negatively charged cartilage-specific glycosaminoglycans in the extracellular matrix. (C) H&E-stained section of the osteogenic layer showing a representative island structure consisting of MSC-differentiated osteoblast-like cells on the surface and in the center. (D) Positive toluidine blue staining of an island structure in the osseous layer.
J DENT RES, Vol. 82, No. 12,
951-956 (2003)
DOI: 10.1177/154405910308201203
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