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Figure 4. Localization of hBD-2 peptide in stimulated HGECs. HGECs grown on coverslips were unstimulated (A) or stimulated overnight with 10 µg of F. nucleatum cell wall extract/mL (B,C), 1.20 mM extracellular calcium (D), or 1000 nM thapsigargin (E). HGECs were fixed and reacted with polyclonal antibody against hBD-2 (A,B,D,E) or pre-immune rabbit serum (C), and fluorescein isothiocyanate-conjugated secondary antibody as previously described (Krisanaprakornkit et al., 2000). Bars represent 40 µm. The data shown are representative of 2 separate experiments.
J DENT RES, Vol. 82, No. 11,
877-882 (2003)
DOI: 10.1177/154405910308201106
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