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Figure 3. HBD-2 mRNA induction by F. nucleatum is dependent on an increase in intracellular calcium. HGECs were pre-incubated with 0, 10, or 30 µM BAPTA-AM for 45 min, and then stimulated with either 10 µg of F. nucleatum cell wall extract/mL for 6 and 12 hrs, 1000 nM thapsigargin, or 1.20 mM calcium for 12 hrs, or were left as unstimulated controls. RT-PCR analysis was performed as described in MATERIALS & METHODS. Note that hBD-2 induction by all 3 inducers was completely inhibited by 30 µM BAPTA-AM.
J DENT RES, Vol. 82, No. 11,
877-882 (2003)
DOI: 10.1177/154405910308201106
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