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Figure 3. Southern blot analysis of real-time RT-PCR products. PCR products with/without reverse transcription (RT) reaction and recombinant plasmids carrying each gtf gene were separated by 2% agarose gel electrophoresis, then transferred onto nylon membranes. The membranes were hybridized with ³²P-labeled gtfB-, gtfC-, and gtfD- specific probes, respectively. Lanes 1, RT-PCR amplicon by gtfB-specific primers; 2, RT-PCR amplicon by gtfC-specific primers; 3, RT-PCR amplicon by gtfD-specific primers; 4, PCR product without RT reaction by gtfB-specific primers; 5, PCR product without RT reaction by gtfC-specific primers; 6, PCR product without RT reaction by gtfD-specific primers; 7, pSK6 carrying gtfB; 8, pSK16 carrying gtfC; and 9, pYT104 carrying gtfD.

J DENT RES, Vol. 81, No. 2, 109-113 (2002)
DOI: 10.1177/154405910208100205

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