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Figure 2. Total RNA and relative quantities of gtfB, gtfC, and gtfD mRNA in the growth phase of S. mutans MT8148. Amounts of extracted total RNA were assessed by means of a RiboGreen RNA quantitation kit with a Fluorometer. Following reverse transcription from 1 µg of total RNA, the amount of each gtf cDNA was determined by real-time PCR. Data are expressed as means and standard deviations of triplicate experiments. Statistical differences (P < 0.05, ANOVA) from early-exponential (*) and mid-exponential (#) phases are indicated.
J DENT RES, Vol. 81, No. 2,
109-113 (2002)
DOI: 10.1177/154405910208100205
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