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Figure 1. Construction and verification of plasmid pCIA-P. (A) The A- and P-regions of PAc protein of S. mutans was cloned into eukaryotic expression vector pCI to obtain the recombinant plasmid pCIA-P. (B) Restriction enzyme digestion of pCIA-P and pCI. The sizes of pCIA-P, A-P fragment, and pCI were 6.2 kb, 2.2 kb, and 4.0 kb, respectively. Lane 1: pCI with XhoI digestion. Lane 2: pCIA-P with Bgl I digestion. Lane 3: pCIA-P with XhoI digestion. Lane 4: pCIA-P with Xho I/Sal I digestion. Lane 5:  DNA/HindIII marker.
J DENT RES, Vol. 81, No. 11,
784-787 (2002)
DOI: 10.1177/154405910208101112
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