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Figure 2. Immunodetection of fibrillin-1, fibrillin-2, and tropoelastin in matrix samples from HGF and HPLF cell layers after 2, 4, and 6 wks of culture. Equal amounts of matrix samples (100 µg) that had been harvested from HGF and HPLF cell layers after 2, 4, and 6 wks of culture were separated by SDS-PAGE and transferred to Immobilon-P membranes. The blots were probed with anti-fibrillin-1 (A), anti-fibrillin-2 (B), and anti-tropoelastin (C) antibodies. To ensure that equivalent amounts of the extracted proteins were present in each lane, we stained another membrane with Amido Black 10B reagent before hybridization (lower panel in A, B, and C). The results are representative of the three independent experiments. The positions of the size markers are indicated on the right. Densitometric scanning of the bands was performed with the use of National Institutes of Health imaging software.
J DENT RES, Vol. 81, No. 11,
771-775 (2002)
DOI: 10.1177/154405910208101110
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