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Figure 4. TNF- stimulates the IL-6 gene promoter via p38 MAPK, NF B, and C/EBPβ. (Panel A) MG-63 cells were transiently transfected with respective plasmid (phIL-6 wild-type [phIL-6WT] or 5'-deletion constructs [phIL-6A and phIL-6B]), serum-starved for 24 hrs, and then stimulated with TNF- (10 ng/mL) for 24 hrs. p38 MAPK inhibition was achieved by pre-incubation with SB203580HCl (10 µM) for 1 hr immediately prior to TNF- stimulation (10 ng/mL). Luciferase activity within cell lysates was measured and corrected for transfection efficiency. *Statistically significant difference from TNF--stimulated cultures (p < 0.05). Bars represent mean ± standard deviation (n = 3), relative to control (unstimulated cultures). (Panel B) TNF- modulates NFB- and C/EBPβ-sensitive promoters via p38MAPK. Cells were transiently transfected with respective plasmid (or vector only), serum-starved for 24 hrs, and then stimulated with TNF- (10 ng/mL) for 24 hrs. p38 MAPK inhibition was achieved by pre-incubation with SB203580HCl (10 µM) for 1 hr immediately prior to TNF- stimulation. Luciferase activity within cell lysates was measured and corrected for transfection efficiency. *Statistically significant difference from control (unstimulated) cultures (p < 0.05). Bars represent mean ± standard deviation (n = 4).
J DENT RES, Vol. 81, No. 1,
17-22 (2002)
DOI: 10.1177/154405910208100105
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